›› 2012, Vol. 24 ›› Issue (4): 283-285,.doi: 10.3969/j.issn.1004-616x.2012.04.010

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Combined toxicity of nitrobenzene and aniline in primary culture of rat hepatocytes

JIA Li;WANG Yan-jun;DONG Shu-ying;SHANG Lei;NA Xiao-lin   

  1. ( 1. Department of Public Health, Harbin Medical University, Harbin 150081; 2. Department of Public Health Surveillance, Center for Disease Control and Prevention of Heilongjiang, Harbin 150030, Heilongjiang, China)
  • Received:2011-09-14 Revised:2011-12-21 Online:2012-07-30 Published:2012-07-30
  • Contact: NA Xiao-lin

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Abstract: OBJECTIVE: To study the combined toxicity of nitrobenzene and aniline on primary culture hepatocyte of Wistar rats. METHODS：Nitrobenzene and aniline alone or combined were used to treat the cells for 24 h，and then MTT method was used to measure the survival rate of the cells. Kits were used to measure the MDA，ALT，SOD，GSH and GSH-px levels in the cells or supernatant. RESULTS：After treating with different concentrations of nitrobenzene and aniline alone or combined for 24 h，the survival rate of the cells was significantly lower than that of the cells in control group，with a dose-response relationship. After treating with 10 and 20 mmol/L nitrobenzene and aniline alone or combined for 24 h，the cell MDA and ALT levels of all groups were significantly higher than that of control group (P<0.05). The cell SOD，GSH and GSH-px levels in groups were significantly lower than that of control group (P<0.05). There were significant differences of MDA，ALT，SOD，GSH and GSH-px levels between the combined treatment group and those treated with nitrobenzene or aniline (P<0.05). CONCLUSION： Nitrobenzene and aniline alone or combined may lead to synergistic toxicity effect on hepatocytes. Further research is needed for study.

Key words: nitrobenzene, aniline, combined toxicity, oxidative damage